Fig. 2: Location of ARK1 during P. falciparum asexual division. | Nature Communications

Fig. 2: Location of ARK1 during P. falciparum asexual division.

From: Plasmodium ARK1 regulates spindle formation during atypical mitosis and forms a divergent chromosomal passenger complex

Fig. 2: Location of ARK1 during P. falciparum asexual division.The alternative text for this image may have been generated using AI.

a Schematic of asexual division during P. falciparum blood stage schizogony. HA-PfARK1-loxP parasites were synchronised and IFA was performed using anti-HA (green) and anti-centrin (magenta) antibodies on schizonts. DNA was stained with DAPI. PfARK1 was observed transiently as a punctum only at nuclei with duplicated centrin foci. Scale bar = 2 μm. b Ultrastructure expansion microscopy (U-ExM) was performed on HA-PfARK1-loxP schizonts using anti-HA (green) and anti-tubulin (magenta) antibodies. Hemispindles (white arrow), mitotic spindles (cyan arrow) and interpolar spindles (yellow arrow) that correspond to different stages of nuclear division were observed. PfARK1-positive puncta were mainly associated with mitotic spindles with the puncta flanking the spindle (square and magnification in the inset). Scale bar = 2 μm. c Percent nuclei that exhibited PfARK1 staining (panel a) were quantified (SEM  ±  SE, two-sided unpaired t test, ***P  = 0.0005, n = 37 parasites, three independent biological replicates. d Nuclei exhibiting PfARK1 foci (panel b) in association with hemispindles, mitotic spindles or interpolar spindles were quantified and represented as % of total nuclei (SEM  ±  SE, one-way ANOVA, ****P  <  0.0001, n > 40 parasites, three independent biological replicates). Source data are provided as a Source Data file.

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