Fig. 4: SFPQ controls DAXX localization and deposition of histone H3.3.
From: SFPQ directs histone H3.3 deposition to R-loops in DNA repeats to protect genome stability
A Pie chart representing percentage of SFPQ-DAXX common binding sites across indicated genome regions in U-2 OS cells transfected with control siRNAs (Control). Right panel, common SFPQ-DAXX peaks in control cells. B Pie chart representing the percentage of DAXX peaks in indicated genome regions in SFPQ depleted U-2 OS cells (loss of SFPQ). C Box-plot representing H3.3 mean Log2 fold change at reported genomic regions in control (blue) and SFPQ depleted (yellow) U-2 OS cells. N, number of peaks for reported genomic region for both conditions. P-values were calculated with Wilcox test and adjusted for False Discovery Rate. Center, median (50th percentile); box bounds, Q1 (25th percentile) and Q3 (75th percentile); whiskers, minimum and maximum non-outlier values (within 1.5 × IQR of Q1/Q3); dots, outliers. Promoter-TSS Promoter-Transcription Starting Site, CpG CpG islands, TTS Transcription Termination Site, LINE Long Interspersed Nuclear Element, SINE Short Interspersed Nuclear Elements, LTR Long Terminal Repeat. Chromatin immunoprecipitation (ChIP) assay performed on U-2 OS cells transfected with indicated siRNAs. Data for (peri)centric and (sub)telomeric repeats (D) transposable elements (E) and positive control regions for SFPQ and DAXX are shown. For quantifications shown in (D–F), data represents the mean values, error bars indicate standard deviation. N = number of independent experiments. An unpaired, two-sided Student’s t test was used to calculate statistical significance; p-values are shown. Source data are provided with this paper.
