Fig. 2: FOXJ1 overexpression confers resistance to docetaxel in vitro.

A LNCaP cells with stable expression of FOXJ1 (FOXJ1_OE) or empty vector control (EV) were immunoblotted for FOXJ1 or vinculin. The blot is representative of three independent experiments. B Image of violet purple stained colonies for FOXJ1_OE and EV cells treated with DMSO and indicated concentrations of DTX. The image is representative of three independent experiments. C Colonies for FOXJ1_OE and EV LNCaP cells in the indicated treatment conditions were counted and normalized based on vehicle treated EV controls. Mean and standard deviation in triplicate wells from one of three representative experiments are shown. D Representative cell cycle profiles for FOXJ1_OE and EV cells treated for 24 h with vehicle (DMSO) or indicated concentrations of DTX. E Percentage of cells in G2/M phase for FOXJ1_OE and EV cells treated with DMSO or indicated concentrations of DTX for 24 h, mean +/− SD, n = 3 independent experiments, * p < 0.05 (p = 0.026 and p = 0.0075, Student’s t-test, two-tailed) compared to EV. F FOXJ1_OE and EV cells were treated with docetaxel for 24 h and immunoblotted for H3S10 phosphorylation. The blot is representative of three independent experiments. G Tubulin polymerization assay showing amount of α-tubulin in pellet fraction (P) and soluble fraction (S) in representative experiment. The P% was calculated based on grayscale intensity of western blot bands for each treatment group [P% = P/( P+ S)*100%]. The blot is representative of three independent experiments. H P% values from three biological repeats for the indicated treatment group in FOXJ1_OE and EV cell, mean +/− SD, * p < 0.05 (p = 0.019 and p = 0.034, Student’s t-test, two-tailed), compared to EV. I Western blot demonstrated the amount of α-tubulin from pellet fraction (P) and soluble fraction (S) in FOXJ1_OE versus control cells after cabazitaxel (CAZ) treatment. The blot is representative of three independent experiments. Source data are provided as a Source Data file.