Fig. 4: Cumulative frequency analysis of load versus dwell time in ATP vs ATPgS.
From: ATPγS substantially defeats the biasing mechanism for kinesin steps
a–d Normalized 1-CDF (%) plots of step dwell times for forward and backward steps across different conditions, fitted with single exponentials. a 1 mM ATP, cumulative distribution plots for bins 2.5–3.5 pN (left) and 7.5–8.5 pN (right), with the corresponding plot of mean dwell time (τ = 1/λ) vs. load (middle, fitted: 4–7 pN), dwell (forward) = 0.0018 e0.62 * load and dwell (backward) = 0.0049 e0.58 * load. b 1 mM ATPγS, τ vs. load plot (fitted: 2–6 pN) dwell (forward) = 0.3619 e0.15 * load. c 1 μM ATP, τ vs. load plot (fitted: 3-7 pN), dwell (forward) = 0.0971 e0.43 * load and dwell (backward) = 0.3895 e0.38 * load. d 1 µM ATPγS, τ vs. load plot (fitted: 2–5 pN), dwell (forward) = 0.4430 e0.31 * load. e, f step amplitude distributions for ATP (1 mM, n = 814) and ATPγS (1 mM, n = 747) steps obtained at superstall loads after a stage-step. g, h same but only for the initial step that followed each stage-step. n = 213 for f and n = 233 for h.
