Fig. 3: Genetic deletion of neutrophil Slc8a1 does not alter neutrophil development, maturity and migration. | Nature Communications

Fig. 3: Genetic deletion of neutrophil Slc8a1 does not alter neutrophil development, maturity and migration.

From: NCX1 reverse mode promotes calcium-dependent Neutrophil Extracellular Trap formation and lung damage in chronic obstructive pulmonary disease

Fig. 3: Genetic deletion of neutrophil Slc8a1 does not alter neutrophil development, maturity and migration.The alternative text for this image may have been generated using AI.

a Flow cytometry analysis of bone marrow neutrophil proportions. b, c The morphology of bone marrow-derived neutrophils was visualized by Diff-Quik staining and scanning electron microscope. Scale bar = 20 µm (b), 2 µm (c). d–f The development and maturation of bone marrow neutrophils, as well as the proportions of each subpopulation (n = 5 mice). g The number of bone marrow neutrophils in the tibia of mice with different genotypes (n = 6 mice). h, i The number of white blood cells and neutrophils in peripheral blood (n = 6 mice). j The proportion of neutrophils to CD45+ cells in lung tissues (n = 6 mice). Each data point represents one biologically independent replicate (e–j). Neutrophils were derived from the bone marrow, blood, and lung tissues of mice with non-CS exposure. All quantitative data shown here are presented as Mean ± SD. A two-sided t-test was used to calculate the p-values for all quantitative data. At least 3 times, each experiment was independently repeated with similar results. Source data are provided as a Source Data file. MB myeloblast; PM promyelocyte; MC myelocyte; MM metamyelocyte; BC band cell; SC segmented neutrophil; BM bone marrow; PB peripheral blood; PMNs polymorphonuclear neutrophils; ns no significance.

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