Fig. 4: Slc8a1 deletion does not affect the phagocytosis capability of neutrophils. | Nature Communications

Fig. 4: Slc8a1 deletion does not affect the phagocytosis capability of neutrophils.

From: NCX1 reverse mode promotes calcium-dependent Neutrophil Extracellular Trap formation and lung damage in chronic obstructive pulmonary disease

Fig. 4: Slc8a1 deletion does not affect the phagocytosis capability of neutrophils.The alternative text for this image may have been generated using AI.

a Flow cytometry analysis shows the purity of neutrophils isolated from the bone marrow of Slc8a1f+/f+;Mrp8-Cre− and Slc8a1f+/f+;Mrp8-Cre+ mice. b, c Flow cytometry analysis shows the phagocytosis of neutrophils to E. coli bioparticles at different time points. d In vitro phagocytosis assay. Purified neutrophils were stimulated with pHrodo Red-conjugated Escherichia coli bioparticles for 2 h and photographed by confocal microscopy. Scale bar = 5 µm. e, f Quantification of phagocytosis efficiency and index between two genotype mice (n = 5) at 30, 60, 120 and 240 min. Each data point represents one biologically independent replicate (e, f). All quantitative data are presented as Mean ± SD. A two-sided t-test was used to calculate the p-values for all quantitative data. At least 3 times, each experiment was independently repeated with similar results. Source data are provided as a Source Data file. BF bright field; min minutes; ns no significance.

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