Fig. 4: Pan2 deletion resulted in disordered mRNA tail length.
From: PAN2 maintains mRNA poly(A) tail homeostasis and regulates translation during spermiogenesis in mice
a Schematic diagram of Smart-seq2 and PAIso-seq sequencing. b, c Volcano plots show the number of significantly differentially expressed genes (DEGs) in MII phase spermatocytes (b) and RS (c) from WT and Pan2fl/–;Stra8-Cre mice. The P threshold (0.05) and log2FC threshold (± 1) are reported in gray horizontal and vertical lines, respectively. FC, fold change. n = 3 biological replicates per group. Two-sided Student’s t-tests. d Sankey diagram showing the flow patterns of downregulated, upregulated, and stable transcripts in RS from WT and Pan2fl/–;Stra8-Cre mice. Each rectangle represents a gene category. FC = ± 2. n = 3 biological replicates per group. e Distribution graph of mRNA poly(A) tail length of upregulated and stable transcripts in Pan2-deleted versus WT RS. n = 3 biological replicates per group. f Violin diagram of mRNA poly(A) tail length of upregulated and stable transcripts in Pan2-deleted versus WT RS. ****P < 0.0001 by two-tailed Student’s t-test. g,h Distribution graph of mRNA poly(A) tail length in MII phase spermatocytes (g) and RS (h) in WT and Pan2fl/–;Stra8-Cre mice. n = 3 biological replicates per group. i Venn diagram displaying the overlapping genes detected in WT and Pan2-deleted spermatocytes at the MII stage. The overlap represents genes with non-zero poly(A) tail lengths in both WT and Pan2-deleted MII phase spermatocytes, which were selected for further analysis of the polyadenylation changes in (j). n = 3 biological replicates per group. j Scatter plot comparing the poly(A) tail lengths of mRNAs in Pan2-deleted versus WT MII phase spermatocytes. Results are from three independent experiments. k Venn diagram displaying the overlapping genes detected in WT and Pan2-deleted RS. The overlap represents genes with non-zero poly(A) tail lengths in both WT and Pan2-deleted RS, which were selected for further analysis of the polyadenylation changes in (l). n = 3 biological replicates per group. l Scatter plot comparing the poly(A) tail lengths of mRNAs in Pan2-deleted versus WT RS. n = 3 biological replicates per group. m Scatter plot comparing DPGs in Pan2-deleted versus WT RS and DPGs in WT RS versus MII phase spermatocytes. n = 3 biological replicates per group. n Scatter plot comparing DPGs in Pan2-deleted versus WT RS and MII phase spermatocytes. n = 3 biological replicates per group.
