Fig. 5: Proteomics analysis verified that PAN2 participated in the regulation of translation during spermatogenesis.
From: PAN2 maintains mRNA poly(A) tail homeostasis and regulates translation during spermiogenesis in mice
a Venn diagram displaying the overlapping proteins detected in WT and Pan2-deleted RS. The overlap represents proteins with non-zero quantity in both WT and Pan2-deleted RS, which were selected for further analysis in (b). b Volcano plot shows the downregulated and upregulated proteins in Pan2-deleted RS. The P threshold (0.05) and FC threshold (± 2.5) are reported in gray horizontal and vertical lines, respectively. FC, fold change. Two-sided Student’s t-tests. c Gene Ontology (GO) analysis of downregulated proteins in Pan2-deleted RS. Two-sided Student’s t-tests. d Heat map showing the expression level of downregulated proteins in WT and Pan2-deleted RS. e Proportion plot showing the proportion of deadenylation and polyadenylation transcripts in the downregulated proteins in the WT and Pan2-deleted RS. f Heat map showing the poly(A) tail length of partially overlapping genes in (e). g, h Poly(A) tail length of transcripts in the WT and Pan2-deleted RS were evaluated using poly(A) tail assays. i, j Relative signal intensity (y-axis) and PCR product length according to mobility (x-axis).
