Fig. 6: PAN2 interacted with PABPC1, and longer poly(A) tails bound more PABPC1.

a Schematic representation of immunoprecipitation coupled with mass spectrometry (IP-MS) analysis of PAN2 in the testis. b Venn diagram displaying the overlapping proteins detected in the IgG and endogenous PAN2 IP groups. The overlap represents proteins with non-zero intensity in both groups, and these were selected for further analysis of protein levels in (c). Results are from two independent experiments. c The scatter plots showing the fold relationship between the PAN2 IP group and the IgG group. The FC threshold (±2) is indicated by gray slashes. FC, fold change. Results are from two independent experiments. d GO analysis of potential PAN2-interacting proteins, as determined using IP-MS. Two-sided Student’s t-tests. e List of some potential PAN2-interacting proteins related to translation and mRNA processing. f Endogenous immunoprecipitation results showing interactions of PAN2 with translation-related proteins in the RS. g Co-immunoprecipitation results showing the interactions between HA-tagged PAN2 and FLAG-tagged PCBP1, with or without RNase A digestion. h Western blotting analysis of EIF5A, EIF4A1, EIF4E, and PABPC1 protein levels in WT control and Pan2^fl/–;Stra8-Cre RS. GAPDH was used as a loading control. i RNA pull-down results showing PABPC1 protein levels bound by probes with different lengths of poly(A) tails. Representative results shown in (g, i) were obtained from at least three independent experiments with similar results. Source data (g,i) are provided as a Source data file.