Fig. 7: Pan2 deletion results in compromised mRNA translation efficiency during the RS stage.
From: PAN2 maintains mRNA poly(A) tail homeostasis and regulates translation during spermiogenesis in mice
a Ribo-lite sequencing schematic diagram. b The scatter plot showing the gene distribution of TE upregulation (FC ≥ 1.5) and TE downregulation (FC ≤ 0.667). FC, fold change. n = 3 biological replicates per group. c Gene Ontology (GO) analysis of TE downregulation in Pan2-deleted RS. Two-sided Student’s t-tests. d The scatter plot showing the correlation between mass spectrometry and Ribo-lite results. e The volcano plot showing that PABPC1 binds to RNA(FC > = 3, P-value < 0.05), and the results are from RIP-qPCR. FC, fold change. Two-sided Student’s t-tests. The data were calibrated using Gapdh. n = 3 biological replicates per group. f Line chart showing the translation efficiency of PABPC1 target genes in WT and Pan2-deletion RS. The middle orange line indicates the median expression level. n = 3 biological replicates. g Violin plot showing the poly(A) length of the PABPC1 target genes and non-target genes in WT RS. ****P < 0.0001 by two-tailed Student’s t-test. h Pie chart showing the proportion of Pan2 target DPGs among the PABPC1 target genes. n = 3 biological replicates. Representative results shown in panels (f–i) were obtained from at least three independent experiments with similar results. Source data (f–i) are provided as a Source data file.
