Fig. 2: trs rice lines showed increased yield related parameters.

a–c Phenotypes of ZH11, trs3 and trs8 plants grown in Wuhan in 2023. Scales bar represent 10 cm (a, b) and 5 cm (c). d–p Yield-related traits of ZH11and trs mutant lines cultivated in Wuhan in 2023 (d–k) and Sanya in 2024 (l–p). Measured parameters include: grain yield per plot (d, l), yield per plant (h, m), panicle length (i, n), grain number per panicle (j, o), yield per panicle (k, p). e, f Statistical analysis of plant height (e), tiller number (f) and panicle number per plant (g) of ZH11, trs3 and trs8 lines cultivated in Wuhan (2023). Panicle-related data were recorded for the main panicle of individual plants. In the bar chart, bars represent the mean ± SD, whereas in the box plot, whiskers represent the data range and the center line represents the median. In both plots, error bars denote variability among biological replicates, and gray dots indicate individual samples. Data in (d) (n = 3 plots, 50 plants within a plot), in (e–g) (ZH11, n = 20; trs3, n = 20; trs8, n = 20), in (h) (plot1, n = 47 per line; plot2, n = 45 per line; plot3, n = 50 per line), in (i) (plot1, n = 44 per line; plot2, n = 45 per line; plot3, n = 49 per line), in (j) (plot1, n = 45 per line; plot2, n = 47 per line; plot3, n = 48 per line), in (k) (plot1, n = 45 per line; plot2, n = 48 per line; plot3, n = 47 per line), in (l) (n = 3 plots, 30 plants within a plot), in (m) (plot1, n = 27 per line; plot2, n = 29 per line; plot3, n = 29 per line), in (n) (plot1, n = 26 per line; plot2, n = 29 per line; plot3, n = 28 per line), in (o) (plot1, n = 28 per line; plot2, n = 28 per line; plot3, n = 27 per line), in (p) (plot1, n = 26 per line; plot2, n = 26 per line; plot3, n = 26 per line). All statistical tests were carried out using a one-way ANOVA, with distinct letters indicating significant differences (P < 0.05). Source data are provided as a Source Data file.