Fig. 2: Signal amplification enables sensitivite detection of low abundance senescence markers in healthy pancreas. | Nature Communications

Fig. 2: Signal amplification enables sensitivite detection of low abundance senescence markers in healthy pancreas.

From: iCLAP: an innovative method for integrable co-detection of low-abundance antigens with high-plex immunostaining

Fig. 2: Signal amplification enables sensitivite detection of low abundance senescence markers in healthy pancreas.

a–c Images showing 53BP1 staining on human pancreatic tissue using TSA-based immunofluorescence (TSA) with a 1:1000 diluted antibody and 40-min incubation (a), indirect immunofluorescence (IF) with a 1:1000 diluted antibody and 40 min incubation (b), and indirect immunofluorescence with a 1:50 diluted antibody and 12 h incubation (c). Corresponding H&E images from adjacent sections of the same field are also included. d P21 staining of 2 h incubation of 1:1000 diluted antibody and 12 h with 1:50 diluted antibody with TSA and IF, respectively. e P53 staining (red) of 2 h incubation of 1:1000 diluted antibody and 12 h with 1:50 diluted antibody with TSA and IF, respectively. f, g Histograms show the distribution of cell P16 intensity in islet and acini regions using indirect immunofluorescence staining (f) or TSA-based immunofluorescent (g). All immunofluorescence images shown are representative of independent staining experiments performed on three independent human pancreatic tissue samples (SENPAN_1, SENPAN_2, and SENPAN_3; see Supplementary Data 1), with similar results observed. The pseudocolor intensity scale bar shown at the bottom right indicates fluorescence intensity values (arbitrary units).

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