Fig. 5: Analysis of senescence and structural markers in pancreatic acini using multiplexed iCLAP-CODEX and iCLAP-IMC. | Nature Communications

Fig. 5: Analysis of senescence and structural markers in pancreatic acini using multiplexed iCLAP-CODEX and iCLAP-IMC.

From: iCLAP: an innovative method for integrable co-detection of low-abundance antigens with high-plex immunostaining

Fig. 5: Analysis of senescence and structural markers in pancreatic acini using multiplexed iCLAP-CODEX and iCLAP-IMC.

a Tissue imaging workflow using a 43-plex iCLAP-CODEX panel, composed of 3-plex iCLAP (P21, P53, P16) combined with a 40-plex CODEX antibody panel. Markers indicated with an asterisk (*) originate from iCLAP staining. Images display staining of P21*, P53*, P16*, panCK, E-cadherin, Ki67, CD45, α-SMA, and EpCAM in tissue sections, alongside an adjacent H&E-stained image. b Representative images illustrating that P21+ cells are typically panCK+, whereas P53+ cells are generally panCK-. c Quantitative analysis showing that 10% of P53+ cells are panCK+, while 65% of P21+ cells are panCK+, indicating distinct epithelial associations of these senescence markers; fluorescence intensities are reported in arbitrary units. d Tissue imaging using a 31-plex iCLAP-IMC panel, comprising a 3-plex iCLAP panel (P21, P53, P16) combined with a 28-plex IMC panel. Representative images highlight colocalization patterns across different markers. The staining results also highlight the spatial association of P21 and panCK.

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