Fig. 1: LARP6 harbours two disordered RNA-binding regions.
From: An intrinsically disordered region mediates RNA-binding selectivity and cellular activities of LARP6
a IP-OOPS workflow (Created with modifications using BioRender. Mardakheh, F. (2026) https://BioRender.com/16v6db4). The detailed protocol is described in Material and Methods and in the text. Star: crosslinking site; red: protein; blue: RNA. b Schematic representation of the RNA-binding regions identified in LARP6 via IP-OOPS. The IP-OOPS-identified RNA-binding regions are marked by stars. LARP6 domains and regions are represented to scale, with numbers denoting the indicated residues at the start and end of each indicated region (LaM: La-motif; RRM1: RNA Recognition Motif 1; SUZ-C: SUZ domain-containing protein 1 C-terminal). The LaM and the RRM1 comprise the La-module, which together with the N-terminal region (NTR) constitute the N-terminal Domain (NTD). The SUZ-C and its adjacent N-terminal segment comprise the C-terminal region (CTR). c IP-OOPS-identified peptides and their N- or C-terminal flanking candidate RNA-crosslinked regions. The identified crosslink-adjacent peptides are retained in the interface of the 1st OOPS due to their proximity to the RNA crosslink sites but are released in the 2nd OOPS from the crosslink sites following trypsin digestion and detected by mass spectrometry. d Computational disorder prediction for human LARP6 protein sequence. The web server IUPred2A (https://iupred2a.elte.hu/)20,65 was used to predict the likelihood of disorder for LARP6, based on ANCHOR2 or IUPred2 algorithms. Higher disorder scores denote higher likelihoods of the protein region being intrinsically disordered. The IP-OOPS-identified RNA-binding regions are marked by stars.
