Fig. 2: The La-module is essential for the RNA-binding activity of LARP6 in living cells.
From: An intrinsically disordered region mediates RNA-binding selectivity and cellular activities of LARP6
a Schematic diagram of the iCLIP workflow (Created with modifications using BioRender. Mardakheh, F. (2026) https://BioRender.com/16v6db4). b Total number of iCLIP peaks (representing distinct binding sites), which were identified for each myc-LARP6 variant. The ∆NTD and ∆La-module mutants did not bind to RNA in cells and were therefore excluded from further downstream analysis. c iCLIP binding profile of indicated myc-LARP6 variants in the 5’UTR of COL1A1 mRNA, as well as the 5’UTR of RPLP2 ribosomal protein mRNA. The identified peak regions with respect to the previously known sites of binding are marked. d Normalised distribution of iCLIP peaks across different genomic regions. The peak numbers were normalised to the length of each genomic region to calculate peak density. LARP6 mostly binds to coding transcripts, with the majority of binding occurring in the 3’UTR regions. e GO cellular component category enrichment analysis of the FL, ΔNTR, and ΔCTR myc-LARP6-bound transcripts. The iCLIP data was normalised to total RNA-seq data to account for transcript abundance.
