Fig. 3: The N-terminal IDR of LARP6 fine-tunes its RNA-binding activity in living cells.
From: An intrinsically disordered region mediates RNA-binding selectivity and cellular activities of LARP6
a Precision-Recall curves for the ΔCTR Pythia model, applied to the FL, ΔNTR, and ΔCTR iCLIP datasets. Area under the Precision-Recall curve (auPRC) values were calculated as measures of the model’s performance in predicting the RNA-binding sites for each dataset. b Precision-Recall curves for the ΔNTR Pythia model, applied to the FL, ΔCTR, and ΔNTR iCLIP datasets. AuPRC values were calculated as measures of the model’s performance in predicting the RNA-binding sites for each dataset. c Comparisons of sequence to structure contributions toward the Pythia model performances for the FL, ΔNTR, and ΔCTR iCLIP datasets. Log2 of DeepLIFT randomised sequence to structure ratio values were plotted (n = 151 (FL), 147 (ΔNTR), and 111 (ΔCTR)). Boxplots mark the lower and upper quartiles. Horizontal lines mark the median. Whiskers extend to the minima and maxima, excluding outliers, which are individually marked. Significance was calculated using a two-sided Wilcoxon rank-sum test. The p-values are reported on the plots. d Metagene analysis of the iCLIP binding sites from myc-LARP6 FL and ΔCTR. The median of normalised crosslink densities observed in the 50 nt surrounding each peak summit are displayed. The shaded regions represent the 95% confidence interval. e Metagene analysis of the iCLIP binding sites from myc-LARP6 FL and ΔNTR. The median of normalised crosslink densities observed in the 50 nt surrounding each peak summit are displayed. The shaded regions represent the 95% confidence interval. f Positional motif analysis of FL myc-LARP6 iCLIP contact sites by PEKA26. Significantly enriched 5-mer motifs in the 100 nt surrounding the crosslink sites in the whole genome are displayed. g Positional motif analysis of FL myc-LARP6 iCLIP contact sites by PEKA26. Significantly enriched 5-mer motifs in the 100 nt surrounding the crosslink sites in the 3’UTR regions are displayed. h Positional motif analysis of ΔNTR myc-LARP6 iCLIP contact sites by PEKA26. Significantly enriched 5-mer motifs in the 100 nt surrounding the crosslink sites in the whole genome are displayed. i Positional motif analysis of ΔNTR myc-LARP6 iCLIP contact sites by PEKA26. Significantly enriched 5-mer motifs in the 100 nt surrounding the crosslink sites in the 3’UTR regions are displayed.
