Fig. 4: NTR provides binding selectivity to the La-module, in vitro.
From: An intrinsically disordered region mediates RNA-binding selectivity and cellular activities of LARP6
a The iCLIP binding profile of FL and ∆NTR myc-LARP6 in the 3’UTR of CTNNA1 mRNA. The 25 nt-long RNA oligonucleotides that are designed to match either the two main FL peaks (P1 and P2) or the 4 peripheral ∆NTR peaks (U1, U2, D1 and D2), are displayed in light blue. b Steady-state BLI binding analysis of LARP6 NTD to each indicated 5’ biotinylated CTNNA1 3’UTR RNA oligonucleotide. A polyC oligonucleotide was used as control. Error bars = mean ± SD (n = 3 independent biological replicates). c Steady-state BLI binding analysis of the LARP6 La-module to each indicated 5’ biotinylated CTNNA1 3’UTR RNA oligonucleotide. A polyC oligonucleotide was used as control. Error bars = mean ± SD (n = 3 independent biological replicates). d Relative Ka values, calculated from the BLI steady state analysis of LARP6 NTD (B) against each RNA oligonucleotide. Error bars = mean ± SD (n = 3 independent biological replicates). e Relative Ka values, calculated from the BLI steady state analysis of LARP6 La-module (C) against each RNA oligonucleotide. Error bars = mean ± SD (n = 3 independent biological replicates).
