Fig. 2: Three-dimensional structure of VsSemiSWEET in lipid bilayers determined by ssNMR.
From: Diverse regulation of functional dimerization of a sugar transporter by different interfacial lipids
a, b Selected regions of the 2D 13C-13C CORD spectrum of 2-VsSemiSWEET with a mixing time of 500 ms, illustrating the assignments of the long-range intra-monomer correlations. Cross peaks corresponding to long-range restraints (|i - j|> 5, where i and j represent correlated residues) are labeled in red. c Selected regions of the 2D 13C-13C CORD spectrum of 1,3-VsSemiSWEET and the 2D NcaCX spectrum of uniformly-13C, 15N labeled VsSemiSWEET, showing the assignments of the long-range inter-monomer correlations. d Superposition of 2D 13C-13C CORD spectra of 2-13C VsSemiSWEET and spin diluted (2-13C:NA = 1:2) VsSemiSWEET samples. Assigned long-range inter-monomer correlations are labeled. e Superposition of the ten dimer structures of VsSemiSWEET with the lowest energy in lipid bilayers, with RMSD of 0.9 ± 0.3 Å of the backbone atoms. The N-helix (residues 1–17) structure remains unconverged due to the lack of assigned chemical shifts in residues from 1 to 5 and 12 to 17, which are not shown.
