Fig. 6: Functional regulation of VsSemiSWEET by different lipid binding.
From: Diverse regulation of functional dimerization of a sugar transporter by different interfacial lipids
a Molecular structures of the lipids POPG and CDL. b Representative conformations of PG-bound dimeric VsSemiSWEET, illustrating intermolecular residue contacts compared to CDL-bound VsSemiSWEET. c Free energy landscape (FEL) of CDL-bound VsSemiSWEET as a function of the open sizes of the outward gate (dout) and inward gate (din). Here, dout corresponds to the distances between the Cα atoms of residues Y62 on the two protomers, while din corresponds to the center-of-mass (COM) distances between the Cα atoms of residues L42–L44 on the two protomers. Representative structures of the states observed on the FEL are shown. d FEL of PG-bound VsSemiSWEET as a function of the opening size of outward gate and inward gate. e Representative conformations of the outward-open and inward-open states. The helices of the outward-open structure are colored dark gray (TM1), red (TM2), and dark green (TM3). The helices of the inward-open structure are colored light gray (TM1), light orange (TM2), and light green (TM3). f illustration showing that the tails of CDL primarily interact with TM2 of VsSemiSWEET. g Contact probability of bound CDL lipids with the residues of VsSemiSWEET. The black dashed box indicates the region with a high density of contact residues. Source data are provided as a Source Data file.
