Fig. 3: Hunger- and infection-induced SRI-36 acts as a diacetyl receptor in ADF neurons. | Nature Communications

Fig. 3: Hunger- and infection-induced SRI-36 acts as a diacetyl receptor in ADF neurons.

From: Intestinal pathogens override hunger-driven decision-making via immune regulation of central serotonin signaling in C. elegans

Fig. 3: Hunger- and infection-induced SRI-36 acts as a diacetyl receptor in ADF neurons.The alternative text for this image may have been generated using AI.

a Diacetyl-evoked GCaMP6 responses in ADF neurons of unc-13(e51) or unc-13(e51);odr-10(ky32) mutants under the indicated conditions. Left, averaged GCaMP6 signals (solid lines, mean; shaded regions, SEM). Right, comparisons of mean responses during the 30-s stimulation period. p = 0.4287, 0.9069, and 0.9945. b Transcriptomic analysis of published RNA-sequencing datasets. A Venn diagram shows 126 putative chemosensory GPCR genes upregulated during fasting and 1667 genes upregulated during P. aeruginosa infection. Four genes were common: sri-36, srr-6, str-163, and srap-1. c–e Diacetyl-evoked GCaMP6 responses in ADF neurons of worms with the indicated genotypes and conditions. ADF-specific RNAi and rescue were performed using Psrh-142. p = 0, 0, 1, 0.9985, and 0.9662 (c), 0.0001 (d), and <0.0001, 1, 0.9906, 0, 0, and 0.9945 (e). f Escape percentages of fasted or P. aeruginosa-infected worms with the indicated genotypes. WT wild type. p = <0.0001, 0.9903, 0.8333, 0, <0.0001, <0.0001, 0.5497, and 0. g Psri-36::GFP expression in well-fed, fasted, or P. aeruginosa-infected worms. Shown are representative images and comparisons of GFP fluorescence intensity. ADF neurons were labeled with Psrh-142::NLS::mCherry. AU arbitrary units. Scale bar, 20 μm. p = <0.0001, 0.0018, and 0. h Subcellular localization of the SRI-36::GFP translational fusion protein in ADF neurons. The inset shows GFP signals in the ADF cilium (region with dashed outline). Scale bar, 20 μm. A similar pattern was observed in 16 animals. **p < 0.01 and ***p < 0.001 (one-way ANOVA with Tukey’s post hoc test for c, e–g; two-sided unpaired t-test for a, d). Brackets indicate the number of animals tested (a, c–e, g) or independent assays (f). Data are shown as means ± SEM. Source data are provided as a Source Data file.

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