Fig. 1: Experimental overview.
a Genetic context around the amplified unit. The repeated sequences (IS26) surrounding the amplified unit encoding blaSHV, and the regions that are important for Nanopore sequencing and bioinformatic analysis (I-SceI restriction site, flanking regions FR1 and FR2, and control regions CR4 and CR5) are shown. b Plasmid pDA61218_116 and location of the amplified unit, I-SceI restriction site, and the control regions CR1 to CR5. c Outline of workflow for Nanopore sequencing. The I-SceI restriction site is represented by a black triangle. d Nanopore sequencing read coverage comparison over five plasmid control regions (CR1 to CR5; see b). Sequencing coverages were normalized towards coverage at the CR1 locus. Results for sequencing of pDNA that was either digested (dark bars) or not (light bars) by I-SceI prior to Nanopore library preparation are shown.
