Fig. 3: Bimodal HDX of NadA in situ. | Nature Communications

Fig. 3: Bimodal HDX of NadA in situ.

From: Structural dynamics and immunogenicity of the recombinant and outer membrane vesicle-embedded Meningococcal antigen NadA

Fig. 3: Bimodal HDX of NadA in situ.The alternative text for this image may have been generated using AI.

A Differences in HDX between rNadA and OMV-embedded NadA are superimposed on the in-silico model of full-length NadA28. In red: HDX bimodality manifesting in OMV-embedded NadA only with overall increased HDX compared to rNadA. In purple: HDX bimodality in both states, but with OMV-embedded NadA showing a higher intensity for the high mass envelope and a low mass envelope with decreased HDX; or bimodality with appearance of low mass envelope in OMV-embedded NadA. In blue: decreased HDX in OMV-embedded NadA compared to rNadA. In dark gray: no HDX information in OMV-embedded NadA. B Illustrative isotopic distributions upon deuteration of peptides showing differences in HDX bimodality between rNadA and OMV-embedded NadA. From top to bottom: peptide 24–39 (+2), 11 s; peptide 40–58 (+2), 1 min; peptide 40–59 (+2), 11 s; peptide 327–340 (+3), 1 min.

Back to article page