Fig. 4: Nucleus, endoplasmic reticulum, glycosomes, and acidocalcisomes all show organelle interaction with endosymbiont.
A t-SNE representation of the endosymbiont, contact site, and ER clusters, with selected fractional profiles (B). Central lines represent mean, with shaded band representing ± 1 standard deviation. C Manual designations for organelle of origin concerning contact site proteins (Supplementary Data 1I). D Glycosomal fractional profile documenting both association and separation with the endosymbiont confirmed by fluorescent microscopy (E) of intact cells showing endosymbiont (S), associated (Gl1) and free (Gl2) glycosomes. ~500 cells were assessed for representative images. Scale bar (2.5 μm) applies to all fluorescent microscopy images. F Acidocalcisomes show similar fractional profile to that of glycosomes, implying selective association with the endosymbiont, confirmed by transmission electron microscopy (G), showing acidocalcisomes interacting (Al1) or free (Al2) from the endosymbiont. ~50 cells were assessed for representative images. N, nucleus. Scale bar (1 µm) applies to all electron microscopy images.
