Fig. 7: A model showing how mitotic MM-BIR contributes to the formation of chromoanasynthesis. | Nature Communications

Fig. 7: A model showing how mitotic MM-BIR contributes to the formation of chromoanasynthesis.

From: Mitotic microhomology-mediated break-induced replication promotes chromoanasynthesis

Fig. 7: A model showing how mitotic MM-BIR contributes to the formation of chromoanasynthesis.The alternative text for this image may have been generated using AI.

a, b At dysfunctional telomeres or DNA double-strand breaks (DSBs), DNA ends utilise the strand annealing activities of RAD52 and PolĪø to bind to exposed DNA with microhomologies in mitosis and initiate end bridging DNA synthesis by PolĪø. c The DNA synthesis initiated is short but become more processive following polymerase switching to PolĪ“ and the recruitment of processivity factors POLD3, PIF1 and PCNA. dā€“f MM-BIR initiated is highly unstable, leading to frequent inter-chromosomal, inter-strand, or intra-strand/fold-back template switching, and this process appears to continue until the DNA is ligated with another DNA end or stabilised by telomere addition. g, h LIG1/3 is required at the final telomere ligation step and could also contribute to joining discontinuous DNA synthesised on the second strand by PolĪ“.

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