Fig. 2: Docking peptide conjugation.
From: Supramolecular coiled-coil peptide platform for site-specific antibody drug conjugate engineering
A Schematic illustration of azide-modified coil peptide reacting with DBCO-bearing payload B DBCO-PEG4-biotin absorbance spectra change upon conjugation to P4N3. At the beginning of the reaction (0 min), the intact DBCO-moiety absorbs in the UV spectrum with a characteristic maxima at 310 nm and stops absorbing when the structure is rearranged as a result of click conjugation. Conjugation kinetics of DBCO-modified payloads to azide-modified docking peptide P4N3 at room temperature in PBS buffer solution (pH 7.4): C DBCO BP Fluor647, D DM1-PEG4-DBCO, E MMAE-CitVal-PEG4-DBCO, F DBCO-mPEG (Mw ~ 10,000), G DBCO-PEG4-biotin, H DBCO-PEG4-DSPE, I 5’-DBCO-N-polyA15GGG. Data are presented as mean ± s.d., n = 3 biological replicates. J TMB-based HRP activity assay measuring the activity of recombinant HRP-P4 conjugate compared to commercially available native HRP (comHRP). Data are presented as mean ± s.d., n = 3 biological replicates. Source data are provided as a Source Data file.
