Fig. 1: Mutational tolerance and sequence conservation at four positions in HIV-1 CA. | Nature Communications

Fig. 1: Mutational tolerance and sequence conservation at four positions in HIV-1 CA.

From: Recurrent and novel evolutionary pathways drive in vitro HIV-1 lenacapavir resistance with diverse phenotypic consequences

Fig. 1: Mutational tolerance and sequence conservation at four positions in HIV-1 CA.The alternative text for this image may have been generated using AI.

a Schematic of the experimental workflow. Mutant libraries were used to infect MT-4 cells (day 0). At day 2, samples containing ~1% GFP⁺ cells were identified by flow cytometry and expanded to allow virus outgrowth in the absence of LEN. At day 5, GFP⁺ cells were analyzed by flow cytometry, and genomic DNA was harvested for next-generation sequencing (NGS) at day 5 or 7. Created in BioRender. Yamashita, M. (2026) https://BioRender.com/ cqlxtjd. b Sequence logos showing amino acid frequencies among viable mutants identified in this study (left) and among naturally occurring 12,261 HIV-1/SIVcpz strains (right) at the corresponding positions. Source data are provided as a Source data file.

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