Fig. 3: Gut microbial strain‑sharing rates across sibling pairs from different family types.
From: Gut microbiome composition and strain-sharing in multiplex autism spectrum disorder families
The strain‑sharing rate was calculated as (number of shared strains/total sibling pairs) × 100%. Box plots showing strain‑sharing rates in multiplex (ASD‑M), simplex (ASD‑S), and TD sibling pairs. In the box plots, the center line represents the median; box limits indicate the 25th and 75th percentiles; whiskers extend to the maximum and minimum values. A Kruskal–Wallis test indicated significant differences among groups (χ²(2) = 15.098, p = 5.27 × 10−4, ε² = 0.038, [95% CI: 0.005–0.085]). Post hoc pairwise comparisons were performed using two-sided Wilcoxon rank‑sum tests with Benjamini–Hochberg correction. All pairwise differences were significant: multiplex vs. simplex, padj = 0.0378; multiplex vs. TD, padj = 0.0028; simplex vs. TD, padj = 0.0066. ε2: epsilon-squared effect size.
