Fig. 4: Comparison of de novo sequencing results obtained by single-protease and mirror-protease strategies. | Nature Communications

Fig. 4: Comparison of de novo sequencing results obtained by single-protease and mirror-protease strategies.

From: DiNovo enables high-coverage and high-confidence de novo peptide sequencing via mirror proteases and deep learning

Fig. 4: Comparison of de novo sequencing results obtained by single-protease and mirror-protease strategies.The alternative text for this image may have been generated using AI.

a De novo sequencing result on the E. coli datasets. b De novo sequencing results on the yeast datasets. Single-protease spectra were analyzed by GraphNovo, and mirror spectra were analyzed by DiNovo. All(GraphNovo) represents the union of LysN(GraphNovo), LysC(GraphNovo), Lys(GraphNovo) and Try(GraphNovo), and All(DiNovo) for the union of LysC + LysN(DiNovo) and Try+Lys(DiNovo). Green hollow bars indicate all results, while orange solid bars indicate high-confidence (full fragment ion coverage) results. c Venn diagrams of high-confidence amino acids and proteins sequenced by DiNovo and GraphNovo. Source data are provided as a Source Data file.

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