Fig. 6: Comparative transcriptomic analysis of SC83288-treated parasites.

Volcano plots showing differentially expressed genes in trophozoites (25 ± 3 h post-infection) treated with 5 nM (a) or 10 nM (b) SC83288 for 4.5 h, compared with untreated controls. The x-axis represents log₂ fold change and the y-axis shows –log₁₀ false discovery rate (FDR)-adjusted p-values. Differential expression was assessed using two-sided quasi-likelihood tests implemented in edgeR, with p-values corrected for multiple comparisons using the Benjamini–Hochberg procedure. Red dots indicate significantly upregulated genes (p < 0.05, –log₁₀(FDR) > 1.3, and fold change > 1.5). Data represent three independent biological replicates per treatment condition. Source data are provided as a Source Data file.