Fig. 5: Comparison of WNV replication in WNV-infected human cerebral organoids depending on their morphology and course of infection.

Human cerebral organoids were infected with 1 × 10⁴ WNV infectious particles. Results were obtained in two independent experiments, both sampling to at least 14 dpi, whereby one experiment additionally included 21 and 28 dpi. Organoids were morphologically distinguished upon the a absence or b presence of choroid plexus (ChP) structures. In retrospect, organoids were furthermore grouped by the course of infection observed. Type A included organoids reaching their highest viral load until 4 dpi, whereas Type B organoids reached their highest viral load later than 4 dpi. The solid line in the graphs to the left is drawn through median values. The solid lines in the graphs to the right are drawn through the values of the individual organoids. Type A Cer-organoids: n = 2–7 per time point. Type B Cer-organoids: n = 0–9 per time point. Type A ChP-organoids: n = 2–10 per time point. Type B ChP-organoids: n = 0-6 per time point. Limit of detection (LOD): 88.5 × 10² FFU/ml. Source data are provided as a Source Data file. Exact sample sizes are provided in Source Data file.