Fig. 3: Pseudomonas aeruginosa (PA) suppresses neutrophil cuproptosis in mouse lungs.
Male C57BL/6 mice were intranasally inoculated with 50 μL of bacterial suspension of PA strain PAO1 (OD600 = 0.6; 7 × 106 CFU/mL in 0.9% sterile saline) for 1, 3, and 7 days. A Quantitative real-time PCR quantification of phosphodiesterase 4D (PDE4D), Tudor domain-containing protein 9 (TDRD9), and integrin subunit alpha 7 (ITGA7) in lung neutrophils. n = 6. mice per group. B Western blot analysis of PDE4D, TDRD9, and ITGA7 expression in lung neutrophils. n = 6 mice per group. C Western blot profiling of cuproptosis-related proteins (dihydrolipoamide S-acetyltransferase [DLAT], ferredoxin 1 [FDX1], lipoic acid synthetase [LIAS], copper-transporting ATPase A/B [ATP7A/B], solute carrier family 31 member 1 [SLC31A1], and tumor protein p53 [p53]) in neutrophils. n = 6 mice per group. D Immunofluorescence (IF) staining demonstrating co-localization of neutrophil marker LY6G (green) with Fe-S cluster proteins DLAT and FDX1 (red) in lung tissues. Nuclei were counterstained with DAPI (blue). Scale bar: 25 µm. n = 6 mice per group. Data represent median ± interquartile range (IQR), and analyzed by two-sided one-way ANOVA with Tukey’s multiple comparisons test (A–D). Source data are provided as a Source Data file.
