Fig. 2: Statistical analysis of nanopore events for WDR5.
From: Catalytic Proximal Protein Oligomerization as an Anti-Tumor Strategy Targeting WDR5
a Representative ionic current traces depicting the translocation of WDR5 in the presence of mixture 1st-#9, mixture 2nd-#5, and WZ‑1 during screening rounds 1, 2, and 3, respectively. b Variations in the Gaussian fit mean values of WDR5 peak current amplitudes induced by incubation with all grouped compounds across the three screening rounds. c Violin plot showing the distribution of peak currents for WDR5 translocation before and after incubation with mixture 1st-#9(blue, n = 168; purple, n =  197), mixture 2nd-#5(blue, n = 230; purple, n = 143), and WZ‑1 (blue, n = 285; purple, n = 317). P values were calculated using two-sided, paired t-tests (without correction for multiple comparisons). Box plots show the mean (center line), the first and third quartiles (box bounds), and the whiskers extending to the most extreme data points within 1.5× interquartile range (IQR) from the quartiles. d Scatter plot of peak current amplitude versus dwell time for WDR5 in the absence and presence of different compound mixtures. e Molecular structure of WZ‑1. f Time-dependent ionic current traces monitoring WDR5 aggregation. WDR5 (500 nM) was incubated with 5 µM WZ‑1, and ionic currents were recorded for 5 min every 30 min at 250 mV. Traces acquired before incubation and at 60, 120, and 180 min post-incubation are shown. g Scatter plots of peak current amplitude versus dwell time for WDR5 at the indicated time points. h Column scatter plot showing peak current amplitude as a function of incubation time, illustrating the real-time aggregation kinetics of WDR5 in the presence of 5 µM WZ‑1. Source data are provided as a Source data file.
