Fig. 2: Michaelis-Menten and allosteric kinetics for ATP-PRT variants.

Saturation curves for ATP (light yellow – circles), at saturating concentrations of PRPP, Mg²⁺ and K⁺. Saturation curves for PRPP (light blue – squares), at saturating concentrations of ATP, Mg²⁺ and K⁺. Allosteric inhibition (light gray) or activation (light pink) curves for l-histidine (triangles), at saturating concentrations of ATP, PRPP, Mg²⁺ and K⁺. The concentration range used for l-histidine is shown below each plot, spanning µM and mM ranges, depending on the variant used. The data shown are the average of three replicates representative of two independent experiments. Error bars represent the standard error of the mean.