Fig. 1: Study overview and distinct gene expression changes associated with VI in stage I LUAD. | Nature Communications

Fig. 1: Study overview and distinct gene expression changes associated with VI in stage I LUAD.

From: Vascular invasion-associated gene expression is detectable in pre-surgical biopsies of stage I lung adenocarcinoma

Fig. 1: Study overview and distinct gene expression changes associated with VI in stage I LUAD.The alternative text for this image may have been generated using AI.

a Overview of cohorts, sequencing technologies, and analyses utilized in the study. Created in BioRender. Steiner, D. (https://BioRender.com/l1bofp9) is licensed under CC BY 4.0. VI vascular invasion, NST no special type, LMP low malignant potential, BMC Boston Medical Center, LHMC Lahey Hospital and Medical Center. All tumors were selected to be stage I at the time of collection, except for one tumor profiled by stRNA-seq that was upstaged to stage IIA under the 8th TNM edition. b Co-expression heatmap of 474 genes differentially expressed between VI and LMP (FDR < 0.01) in a discovery cohort of stage I LUAD tumors (n = 103) grouped into k = 4 clusters. Heatmap units are log counts per million (CPM) scaled by transcript. LI lymphatic invasion. c Top 10 enrichment terms of each gene co-expression cluster. Pathways were ranked within each cluster by FDR values obtained analyzing each cluster’s gene list with EnrichR and the MSigDB Hallmark 2020 (H) and GO Biological Process 2021 (BP) gene set collections. Source data are provided as a Source data file.

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