Fig. 2: Fra/DCC receptors control protrusion formation on EB that preferentially contact old EC.
From: Frazzled/DCC directs spatial progenitor integration ensuring steady-state intestinal turnover
A–G Confocal images of midguts (R5) after 7 days of kluReDDM-tracing and EB specific manipulations of Fra/DCC and Unc-5 receptors. Scale bar is 50 µm. H Quantification of EB protrusion lengths upon Fra/DCC and Unc-5 manipulations (p = 0.0003 for >unc-5-RNAi; p = 0.0011 for >fragRNA;>hDCC). Confocal images of kluReDDM controls (w1118) (R5) (I) and knockdown of fra (J) showing EB with protrusions targeting to old EC (blue arrows) and to new EC (red arrows). K Quantification of protrusions targeting to old EC and to new EC upon EB specific manipulations of Fra and Unc-5 (p = 0.019767;p = 0.001382;p = 0.177377;p = 0.003663). L Quantification of rEC renewal frequency upon manipulations of Fra/DCC and Unc-5(p = 0,7804 for >fra;p = 0.0003 for >unc-5;p = 0.3158 for >unc-5-RNAi). M Quantification of new EC numbers upon kluReDDM-specific manipulation of Fra/DCC and Unc-5 receptors (p = 0.0282 for >fra-RNAi;p = 0.025 for >unc-5). N, P Kaplan-Meier estimations of survival in flies with kluReDDM-driven manipulations of Fra and Unc-5 receptors. Mean survival time is shown in days (d). L, M For box plots, the boxes show median, 25th and 75th percentiles, and whiskers indicate the range of values. (H) Bar plots show means and range indicated by whiskers. All data points are shown by dots and means are indicated by light blues lines. L–P ‘n’ are numbers of biological replicas and ‘N’ values describe numbers of technical replicas. Asterisks denote significances from multiple comparisons by Kruskal Wallis tests (H, L, M) and Long-rank tests (N, P) (*p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001).
