Fig. 3: The specificity of Rca partitioning. | Nature Communications

Fig. 3: The specificity of Rca partitioning.

From: Partitioning of Rubisco activase into the pyrenoidal Rubisco condensate is mediated by a functional protein-protein interaction

Fig. 3: The specificity of Rca partitioning.The alternative text for this image may have been generated using AI.

a Spectrophotometric Rubisco activase assays were performed using 0.2 µM Rubisco and 5 µM Rca from different species. Cr, Chlamydomonas reinhardtii; Os, Oryza sativa; Ath, Arabidopsis thaliana; At, Agave tequilana; Nt. Nicotiana tabacum; Rs, Rhodobacter sphaeroides; Hn, Halothiobacillus neapolitanus; Af, Acidithiobacillus ferrooxidans. n refers to technical replicates. Error bars represent the mean ± S.D. b Droplet sedimentation assay indicates functionally compatible green Rca proteins partition to the dense phase. c Micrographs of condensates to assess DyLight 405-labelled Rca partitioning. Scale bars: 10 μm. The experiment was performed once (n = 1). d Multiple Rca proteins can form binary condensates with EPYC1. Reactions contain 0.3 µM EPYC1-GFP. Scale bars: 10 μm. The experiment was performed once (n = 1). e Sedimentation assays of the reactions shown in (d). f Densitometric quantification of Rca sedimentation shown in (b), (e) and their technical replicates (n = 3). Error bars indicate the mean and s.d. of the percentage of activase pelleted. Source data are provided as a Source Data file.

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