Fig. 4: Specific N-terminal domain residues dictate Rca partitioning to the Rubiscondensate. | Nature Communications

Fig. 4: Specific N-terminal domain residues dictate Rca partitioning to the Rubiscondensate.

From: Partitioning of Rubisco activase into the pyrenoidal Rubisco condensate is mediated by a functional protein-protein interaction

Fig. 4: Specific N-terminal domain residues dictate Rca partitioning to the Rubiscondensate.The alternative text for this image may have been generated using AI.

a Scheme of Rca indicating the disordered N-terminal domain. b N-terminal domain mutants are functional ATPases but are unable to activate Rubisco. Error bars indicate S.D. of three independent experiments (n = 3). c Sedimentation assay of binary condensates. d Sedimentation assay of ternary condensates. The tested variants do not partition to the dense phase. e Quantification of sedimentation assays shown in (c) and (d) comparing binary and ternary condensates. Error bars represent the mean and S.D. from three different experiments (n = 3). f Micrographs of condensates formed using EPYC1 and the N-terminal domain mutants. Scale bars: 10 µm. Reactions include 0.3 µM EPYC1-GFP. The experiment was performed once (n = 1). Source data are provided as a Source Data file.

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