Fig. 3: A TeA–dPAG circuit determines running behaviours.
a–h Blockade of the TeA–dPAG pathway in mice running on the turntable: TeAdPAG CaMKII neurons (a–d) and TeAdPAG CaMKII neurons (e–h). a, e Virus injection site and chemogenetic (CNO) and optogenetic (yellow light LED) interventions. b, f Virus expression. c, g Representative flight speed trace following random presentation of Sound, Light, and Air puff stimuli before (CNO- or LED-) and after (CNO+ or LED + ) interventions in hM4D(Gi)- or eNpHR-expressing animals. Thick lines: average flight speed (30 trials). d, h Peak flight speeds before vs after CNO or LED intervention. (n = 7 animals/group, ****P < 0.0001, two-way ANOVA with the Bonferroni post hoc correction). i Virus injection strategy for TeAdPAG CaMKII neurons blockade. j Virus expression for (i). k Representative traces before (CNO-) and after (CNO + ) intraperitoneal (i.p.) injections of CNO in hM4D(Gi)-expressing animals. l Performance of hM4D-expressing and control mice in the open field test (i): distance travelled, speed, immobility time, and time spent exploring the centre zone before (CNO-) and after (CNO + ) intraperitoneal injections of CNO. (n = 10 animals/group, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001, two-way ANOVA with the Bonferroni post hoc correction). m–o The same as in (i–k) for TeAdPAG CaMKII neurons blockade. p Same as (l), but for (m). (n = 10 animals/group, ****P < 0.0001, two-way ANOVA with the Bonferroni post hoc correction). c, g Data are presented as mean ± SEM; in all other panels, data are presented as mean ± SD. (Source data are provided as a Source Data file; see Supplementary Data 1 for detailed statistics).
