Fig. 7: Firing and running evoked by the activation of ACTeA and TeAdPAG neurons. | Nature Communications

Fig. 7: Firing and running evoked by the activation of ACTeA and TeAdPAG neurons.

From: An intralayer microcircuit in the temporal association cortex underlies sensory-induced escape in mice

Fig. 7: Firing and running evoked by the activation of ACTeA and TeAdPAG neurons.The alternative text for this image may have been generated using AI.

a, b Schematic of virus injection and experimental protocol. c–e Firing rates of ACTeA neurons expressing ChR2 (c), AC-TeA neurons not expressing ChR2 (d) and TeAdPAG neurons expressing ChR2 (e) that responded to the speed of running behaviours evoked by blue light LED stimulation (20 Hz, 5 s, 10 trials). f, g Firing rate (f, n = 12, 7, and 8 cells, P < 0.0001, one-way ANOVA with the Tukey’s post hoc correction.) and running speed (g, n = 6 animals/group, *P < 0.05, one-way ANOVA with the Dunnett T3 post hoc correction). h Correlations between the firing rate and running speed. ACTeA-ChR2+: 0.8980 ± 0.0737; AC-TeA-ChR2-: 0.8301 ± 0.0879; TeAdPAG-ChR2+: 0.8537 ± 0.0792; n = 12, 7, and 8 cells, respectively. Upper and lower quartiles (box plots), min and max values (whiskers), mean (center line). i Firing latency. (n = 12, 7, and 8 cells, ****P < 0.0001, one-way ANOVA with the Dunnett T3 post hoc correction). j Difference in time between firing events and running events. (n = 12, 7, and 8 cells, ****P < 0.0001, one-way ANOVA with the Bonferroni post hoc correction). ACTeA-ChR2+: 2.419 ± 0.184; AC-TeA-ChR2-: 2.075 ± 0.202; TeAdPAG-ChR2+: 2.078 ± 0.148. k Virus injection and chemogenetic (CNO) and optogenetic (LED) interventions. ACTeA and S1TeA neurons expressing ChR2-EYFP; TeAdPAG neurons expressing hM4D(Gi). l, m Representative LED light (20 Hz, black bar: 5 s stimulus duration)-induced speed heatmap (l) and running speed traces (m) before (Pre), during (CNO), and recovery (Rec) from the effect of i.p. injections of CNO. n Induced peak speed for the Pre, CNO, and Rec periods. (n = 7 animals, ****P < 0.0001, one-way repeated-measures ANOVA with the Bonferroni post hoc correction). o L5 IT and PT neurons in the TeA microcircuit underlying sensory-induced escape. f, g, m Data are presented as mean ± SEM. i, j, n Data are presented as mean ± SD. (Source data are provided as a Source Data file; see Supplementary Data 1 for detailed statistics).

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