Fig. 2: Position tuning in anterior and posterior RSC differs in reliability and spatial scales.
From: Anterior and posterior retrosplenial cortex form distinct visuospatial circuits in the mouse
A Left: Examples of normalized deconvolved calcium activity (∆F/F0) from individual position-tuned RSC neurons, plotted as a function of the animal’s position across trials. Right: Proportion of position-tuned neurons in anterior versus posterior RSC. Gray/black connected lines indicate individual imaging sessions for the same animal (gray: Thy1-GCaMP6s; black: CaMKII-GCaMP6). Vertical bars indicate mean ± SEM. Two-sided Mann–Whitney U test, p = 0.0017; n = 10 sessions per group from 8 animals. B Cross-validated, trial-averaged deconvolved ∆F/F0 activity for all position-tuned neurons in anterior and posterior RSC. Neurons are sorted by the position of peak activity computed from half of the data and plotted with the remaining half (n = 10 sessions). C Distribution of spatial information across position-tuned neurons, mapped onto the dorsal cortical surface. The heatmap shows mean spatial information at each cortical location. Histograms display averages across bins along the anterior–posterior and medial–lateral axes (n = 10 sessions). Cumulative distributions comparing D tuning field width, E spatial information (bits/event), and F trial-to-trial correlation coefficients between anterior and posterior RSC neurons. Animal- and recording-wise mean ± SEM values are shown with connected lines (gray: Thy1-GCaMP6s; black: CaMKII-GCaMP6). Statistical comparisons were performed using two-sided Kolmogorov–Smirnov (KS) tests on pooled data (4728 vs. 2205 neurons) and Two-sided Mann–Whitney U tests on per-session averages (n = 10 sessions): KS test vs. Mann–Whitney U test; tuning field width, p = 1.8e-70 vs. p = 0.002; spatial information, p = 4.2e-62. p < 0.001; median trial correlation, p = 7.6e-46 vs. p = 0.017. G Fraction of neurons with one, two, or three position fields. Vertical bars indicate mean ± SEM across sessions. Two-sided Mann–Whitney tests: 1 field, p = 0.10; 2 fields, p = 0.21; 3 fields, p = 0.006 (n = 10 sessions).
