Fig. 1: M. japonica supplementation increases Bacteroides within the fecal microbiota.
A Percentage abundance change of Bacteroidaceae between diet and study fecal samples. A linear regression model of % composition was generated using microviz64, and a Tukey test used to measure significance (adjusted p value; two-sided)79. B 16S rRNA gene relative abundance of genus’ above 10,000 reads within fecal samples. Diet (Control: n = 9 and M. japonica: n = 9) and Study (ad libitum: n = 10 and Silage: n = 8) are denoted below the heatmap. C MAGs generated and dereplicated between fecal and rumen metagenomes. Rumen MAGs are denoted with an orange circle and fecal with blue. D Volcano plot showing proteins with significantly different protein expression recovered from fecal samples from cattle fed diet with 5% inclusion level of M. japonica vs those fed the control diet (two-sided Student’s T-test; S0 = 0). Difference in protein expression is displayed as Log2 fold change in LFQ intensities. Proteins are colored based on their presence within BxMAGBOV genome and the BxMAGBOV CarPUL. Critical and highly expressed proteins are further labelled. E BxMAGBOV CarPUL detected within M. japonica supplemented cattle. Heatmaps indicate LFQ intensity for each BxMAGBOV CarPUL gene for all four replicates of fecal samples of cattle fed control diet and diet supplemented with 5% M. japonica in the silage study. Genes that do not have a heatmap present were not identified within proteomes. Source data are provided as a Source Data file.
