Fig. 5: Lcn2 deletion reduces proinflammatory cytokine release from microglia following LPS stimulation.
From: DRP1 induces neuroinflammation via transcriptional regulation of NF-ĸB.
a Primary microglia were plated in 96-well plates and treated with LPS (100 ng/ml) for 6 h. Conditioned media were collected and analyzed using MSD multiplex immunoassay. N = 3–5 independent experiments per analyte, with 6 technical replicates per experiment; Data are presented as mean ± SEM, two-way ANOVA followed by Tukey’s post hoc test. b Microglia derived from Lcn2+/+, Lcn2+/−, and Lcn2−/− mice were treated with LPS (100 ng/ml, 6 h) and subjected to ChIP analysis as described. DNA immunoprecipitated with anti–NF-κB p65 antibody or IgG control (“NF-κB p65” or “IgG”) was analyzed by qPCR using primers targeting κB sites within the promoters of Lcn2, Cxcl10, and Il6. Ct values were used for quantitative analysis. Data represent mean ± SEM from 3 independent experiments, three-way ANOVA followed by Tukey’s post hoc test. Source data are provided as a Source Data file.
