Fig. 4: Single-frame live-cell SR imaging with 3Snet-CLID acquired on a WF microscope. | Nature Communications

Fig. 4: Single-frame live-cell SR imaging with 3Snet-CLID acquired on a WF microscope.

From: High-fidelity single-frame computational super-resolution using signal-preserving denoising-enabled deconvolution

Fig. 4: Single-frame live-cell SR imaging with 3Snet-CLID acquired on a WF microscope.The alternative text for this image may have been generated using AI.

a Utilizing 3Snet-CLID to analyze the intricate actin structures within the immune synapse of Jurkat T cells. Top: WF microscopy, sparse-deconvolution, and 3Snet-CLID imaging of F-actin labeled with F-tractin-StayGold in live Jurkat T cells. Bottom: Kymographs of F-tractin-StayGold in the region corresponding to the red line. Zoomed-in regions were shown in top-left. Representative data from four biologically independent replicates are shown. Scale bar: 2 μm. b The actin arcs anisotropy of the peripheral supramolecular activation cluster (pSMAC) in (a) measured by FibrilTool. Compared to WF microscopy and sparse-deconvolution, 3Snet-CLID resolves more clear actin arc structure of the pSMAC, and provides more accurate quantified anisotropy data reflecting the effects of actin arc in the pSMAC organization. c Decorrelation analysis applied to the WF image for resolution estimation. d Decorrelation analysis applied to the 3Snet-CLID image for resolution estimation.

Back to article page