Fig. 5: Single-frame SR imaging with 3Snet-CLID acquired on a SD microscope. | Nature Communications

Fig. 5: Single-frame SR imaging with 3Snet-CLID acquired on a SD microscope.

From: High-fidelity single-frame computational super-resolution using signal-preserving denoising-enabled deconvolution

Fig. 5: Single-frame SR imaging with 3Snet-CLID acquired on a SD microscope.The alternative text for this image may have been generated using AI.

a Representative confocal image of the genome-edited fixed U-2 OS cell line with endogenously labeled Nup96-sfGFP. Representative data from from four biologically independent replicates are shown. Scale bar: 2 μm. b Zoom in regions (1-4) from (a) with 3Snet-CLID reconstruction. Scale bar: 1 μm. c Intensity profiles of the nuclear pore indicated by the arrow heads in region 1 of (b). d Averaged diameters of rings formed by Nup96-sfGFP (103 ± 14 nm; n = 64 from three cells). Data are presented as mean ± S.E.M. For the box-and-whisker plots, the center line represents the median, the box spans the interquartile range (IQR; 25th to 75th percentiles), and whiskers extend to 1.5× IQR. Outliers are plotted as individual points. e Dual color imaging of TOM20-StayGold-labeled mitochrondria (green) and mScarlet3-S2-labeled ER (magenta) in live U-2 OS cells. Scale bar: 5 μm. f Decorrelation analysis applied to the SD and 3Snet-CLID images for resolution estimation. Data are presented as mean ± S.E.M. For the box-and-whisker plots, the center line represents the median, the box spans the interquartile range (IQR; 25th–75th percentiles), and whiskers extend to 1.5× IQR. Outliers are plotted as individual points. n = 9 from three biological replicates. Time-lapse of zoomed-in regions 1–4 from (e), showing the corresponding SD (g) and 3Snet-CLID reconstruction (h) images. Scale bar: 500 nm.

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