Fig. 1: Radiation promoted CXCL13 expression and enhanced CXCR5⁺ monocytes infiltration in tumor.

A–C The proportion and cell number of monocytes in MC38 (A), PanC02 (B), and 4T1 (C) tumors. Tumors received one dose of 12 Gy ironizing radiation (IR) 10 days post tumor inoculation and were removed three or seven days after IR. Cell suspension was analyzed by flow cytometry. For MC38 tumor models, n  =  8 in untreated and IR-d3 groups, n  =  7 in IR-d7 group. For PanC02 tumor models, n  =  7 in untreated groups, n  =  8 in other groups. n  =  5 mice/group for 4T1 tumor model. D The quantitation of chemokines in tumor with or without radiation. n  =  3 biological replicates. E Mean fluorescence intensity staining of tumor tissue. CXCL13 (magenta), nuclear (blue). n  =  5 mice/group. F Immunofluorescence staining of tumor tissue. CD45 (green), Ly6C (gray), CXCR5 (red), nuclear (blue). Data is representative of n  =  3 biological replicates. G The components of CXCR5⁺ population in tumor. n  =  5 mice/group. H The expression of CCR2 and CX3CR1 on tumor infiltrated monocytes. n  =  6 mice/group. I–K The proportion and quantity of CXCR5⁺ monocytes in MC38 (I), PanC02 (J), and 4T1 (K) tumors. For MC38 tumor models, n  =  7 mice/group. For PanC02 tumor models, n  =  7 in untreated groups, n  =  8 in other groups. n  =  5 mice/group for 4T1 tumor model. Statistical analysis by two-sided unpaired t-test (D, E) or one-way analysis of variance (ANOVA) (A–C, G, and I–K). Data are represented as mean ± SD (A–C, G, and I–K); * p < 0.05; **p < 0.01; ***p < 0.001 and NS, not significant.