Fig. 6: Radiation induced human CXCR5+ monocytes infiltration in tumor.
From: CXCR5+ monocyte emigration impairs the radiation-induced antitumor immune response
A Heatmap of the mRNA expression of tumor tissue from pancreatic patients who received radiotherapy or not (GSE129492). B Schematic diagram of human CXCR5+ monocytes induction and trans-well assay. TS, tumor supernatant. PBMC, peripheral blood mononuclear cell. Created with MedPeer (medpeer.cn). C CXCR5 expression on monocytes cultured with 25% TS. D The quantity of migrated monocytes. E THP-1 transfer assay. CXCR5+CTV-labled THP-1 cells in tumor were analyzed. n = 6 mice/group. Created with MedPeer (medpeer.cn). F Monocytes in PB. PB was tested within a 7-day window before and after the radiotherapy initiation. All patients were assessed according to RECIST. n = 13 individuals in partial response (PR) group, n = 14 in stable disease (SD) group, and n = 6 in progressive disease (PD) group. Details are shown in Supplementary Table 4. G Representative CT images of patients before and after radiotherapy. (H and I) Representative immunofluorescence staining of tumor tissue from rectal cancer patients who received radiotherapy. CXCL13 (red), nuclear (blue) (H); CD14 (green), CXCR5 (red), nuclear (blue) (I). n = 3 individuals. J, K CXCR5 expression on monocytes. Monocytes pretreated with 1 μM regorafenib, 10 μM LY294002, and 10 μM rapamycin were cultured with TS (J). Monocytes were cultured with rhVEGFA and rhVEGFC (K). (L) PD-L1 expression on human CXCR5- and CXCR5+ monocytes. M Representative immunofluorescence staining of tumor tissue from rectal cancer patients who received radiotherapy. CD14 (green), PD-L1 (red), nuclear (blue) (I). n = 3 individuals. N–P Tumor growth of MC38 (N), PanC02 (O), and 4T1 (P) tumor–bearing mice treated with IR and regorafenib. n = 5 mice/group. Q Graphical abstract of this study. Created with MedPeer (medpeer.cn). n = 3 cells examined over 2 independent experiments (C, D, and J–L). Statistical analysis by two-sided one-way (C, J, and K), or two-way (L–P) ANOVA, or unpaired (D, E) and paired (F) t-test. Data shown in panels (N–P) are represented as mean ± SEM, other panels are represented as mean ± SD; * p < 0.05; **p < 0.01; ***p < 0.001, and NS, not significant.
