Fig. 3: Biodistribution and anti-tumor efficacy of IPANP combined with laser irradiation in vivo based on primary BC tumor models. | Nature Communications

Fig. 3: Biodistribution and anti-tumor efficacy of IPANP combined with laser irradiation in vivo based on primary BC tumor models.

From: Programmable nanomicelles rewire myeloid immunity for durable control of primary and metastatic breast cancer

Fig. 3: Biodistribution and anti-tumor efficacy of IPANP combined with laser irradiation in vivo based on primary BC tumor models.The alternative text for this image may have been generated using AI.

a In vivo fluorescence imaging of 4T1 tumor-bearing mice at a series of time points after intravenous injection of IR825, IPNP, and IPANP (n = 3 mice per group). b Ex vivo fluorescence imaging of tumors and major organs at 72 h post-injection (n = 3 mice per group). c Schematic illustration of the treatment schedule on the 4T1 primary tumor model. Created in BioRender. Zonghe, L. (2026) https://BioRender.com/48gbeau. d Tumor growth curves after different treatments (n = 6 mice per group). e Survival curves of the 4T1 tumor-bearing mice with different treatment (n = 10 mice per group). f Photographs of tumors excised from 4T1 tumor-bearing mice in different treatment groups on day 15 (n = 6 mice per group). g, h Weight of excised tumors in each group and tumor inhibition rate calculated according to the tumor weight (n = 6 mice per group). i–k Representative images and quantitative analysis of Ki67 and CD31 staining of tumor sections after different treatments (n = 6 mice per group). The scale bar is 100 μm. l Schematic of treatment timeline on the EMT6 primary tumor model. Created in BioRender. Zonghe, L. (2026) https://BioRender.com/48gbeau. m Tumor growth curves of EMT6 tumor-bearing mice with different treatments (n = 6 mice per group). n Survival curves of EMT6 tumor-bearing mice during a 60-day observation period (n = 10 mice per group). o Photos of tumors excised from EMT6 tumor-bearing mice in different treatment groups (n = 6 mice per group). p, q Tumor weight and tumor inhibition rate on the EMT6 primary tumor model in each group (n = 6 mice per group). r–t Immunohistochemical analysis of tumor sections after different treatment (n = 6 mice per group). The scale bar is 100 μm. Statistical significance was determined using one-way ANOVA with Tukey’s multiple comparison tests in (g–i, k, m, p–r, t), and two-way ANOVA with Tukey’s multiple comparison tests in (b, d). Data are shown as mean ± SD (*P < 0.05, **P < 0.01, *** P < 0.001, **** P < 0.0001). Source data are provided as a Source data file.

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