Fig. 2: Interactions between PdNorQ^WB and PdNorD^VWA.

a Sequence logos of pore loop, H2i (helix 2 insert) and NorD fingertip. Exemplary residues involved in NorQD interactions are highlighted in red. The GRXLL/IXXXXT motif is highlighted in yellow. b Hydrogen bonds between the NorQ H2i and the NorD VWA fingertip indicated by pink dashes. The participating residues are shown and listed. Individual chains of NorQ are labelled with capital letters A through F. c Contacts between the NorQ H2i and the NorD VWA fingertip with van der Waals overlap ≥ −0,4 Å are indicated as dashed lines and interacting residues are labelled. Details of contacts are described in Supplementary Tables 3 and 4. d Hydrogen bonds between the NorD C-terminus (CT) and the NorQ postS1 loops. e Copurification of PdNorD with PdNorQ from wild type and from cells that carried deletions of the finger motif (Δfinger) or at the C-terminus (Δ637, Δ638) of NorD or a L83A mutation in NorQ. The percentage of ATPase activity compared to the wild type complex is provided for the Δfinger and Δ638 complexes (n = 3), and the relative amounts of iron insertion into cNOR (compared to wild type NorD) is given for the Δfinger construct (n = 2). nd = not determined. Statistical details are found in Supplementary Tables 5 and 6. Source data are provided as a Source Data file.