Table 2 Overview of the numbers and efficiencies of the different steps of the experimental procedure in the 3 different experimental groups: oocyte injection with low CRISPR component concentration, zygote injection with low CRISPR component concentration, and zygote injection with high CRISPR component concentration
Oocytes low conc.(Group 1) | Zygotes low conc.(Group 2) | Zygotes high conc.(Group 3) | Total | |
|---|---|---|---|---|
Number of oocyte collections (combined OPUs and OvHs) | 11 | 20 | 42 | 73 |
Number of collected oocytes | 317 | 515 | 993 | 1825 |
Number of in vitro matured oocytes (MII) used for injections | 98 | 166 | 374 | 638 |
Number of developed embryos (% cleavage rate) | 11 (11.2) | 54 (32.5) | 196 (52.4) | 261 (40.9) |
Number of transferred embryos | 3 | 22 | 56 | 81 |
Number of embryo transfers (ET) | 2 | 9 | 20 | 31 |
Number of pregnancies; all resulted in deliveries (% pregnancy rate) | 0 (0) | 3 (33.3) | 3 (15) | 6 (19.4) |
Number of newborns (% newborns per ET) | 0 (0) | 4 (44.4) | 4 (20) | 8 (25.8) |
Number of KO animals (% KO per ET) | 0 (0) | 0 (0) | 2 (10) | 2 (6.5) |
