Fig. 2: Effect of culture age on cell division status revealed by mCherry protein dilution.
From: Beyond metabolic dormancy: metabolic rewiring in bacterial persistence
E. coli carrying an inducible mCherry expression system was grown for 16, 24, or 48 h (h) in the presence of inducer and then transferred to fresh medium without inducer. Flow cytometry histograms show mCherry fluorescence at 0, 1, and 2 h after transfer. At 0 h, all cells displayed high fluorescence. As cells grew and divided, their fluorescence decreased due to dilution of mCherry (mCherrylow; dashed lines), whereas non-growing cells retained high fluorescence (mCherryhigh; solid lines). Number of biological replicates, n = 4. All raw data, including biological replicates, are available in the data repository Figshare100.
